Association of Bacterial Communities with Psychedelic Mushroom and Soil as Revealed in 16S rRNA Gene Sequencing.
Karthiyayini Balakrishnan, Dheebhashriee Krishnaa, Gowdhami Balakrishnan, Muthuselvam Manickam, Akbarsha Mohammad Abdulkader, Dhanasekaran Dharumadurai
Applied biochemistry and biotechnology May 1, 2024 Peer reviewed DOI: 10.1007/s12010-023-04527-5 via PubMed
Summary
The study identified the microbial communities associated with the psychedelic mushroom Psilocybe cubensis and its surrounding soil in Kodaikanal, Tamil Nadu, India. High-throughput amplicon sequencing revealed distinct microbial diversity, with the most abundant bacterial genera being Ochrobactrum, Stenotrophomonas, Achromobacter, and Brevundimonas. Environmental and anthropogenic factors significantly impacted the microbiome of both the mushroom and the soil. Further investigation is needed to understand how these bacterial communities influence mushroom growth.
Study at a glance
| Population | Psychedelic mushroom Psilocybe cubensis and its surrounding soil |
|---|---|
| Key finding | Distinct microbial diversity was found in the mushroom and related soil, influenced by environmental and anthropogenic factors. |
Abstract
Microbial communities' resident in the mushroom fruiting body and the soil around it play critical roles in the growth and propagation of the mushroom. Among the microbial communities associated with psychedelic mushrooms and the rhizosphere soil, bacterial communities are considered vital since their presence greatly influences the health of the mushrooms. The present study aimed at finding the microbiota present in the psychedelic mushroom Psilocybe cubensis and the soil the mushroom inhabits. The study was conducted at two different locations in Kodaikanal, Tamil Nadu, India. The composition and structure of microbial communities in the mushroom fruiting body and the soil were deciphered. The genomes of the microbial communities were directly assessed. High-throughput amplicon sequencing revealed distinct microbial diversity in the mushroom and the related soil. The interaction of environmental and anthropogenic factors appeared to have a significant impact on the mushroom and soil microbiome. The most abundant bacterial genera were Ochrobactrum, Stenotrophomonas, Achromobacter, and Brevundimonas. Thus, the study advances the knowledge of the composition of the microbiome and microbial ecology of a psychedelic mushroom, and paves the way for in-depth investigation of the influence of microbiota on the mushroom, with special emphasis on the impact of bacterial communities on mushroom growth. Further studies are required for a deeper understanding of the microbial communities that influence the growth of P. cubensis mushroom.