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The Differentiation of Lysergic Acid Diethylamide (LSD) from N-Methyl-N-Propyl and N-Butyl Amides of Lysergic Acid

Cc Clark

Journal of Forensic Sciences May 1, 1989 Peer reviewed DOI: 10.1520/jfs12674j

Summary

Electron impact mass spectroscopy (EI/MS) combined with other analytical techniques can effectively differentiate lysergic acid diethylamide (LSD) from various synthesized amides of lysergic acid. The study involved the synthesis of several N-substituted amides, which were then separated and analyzed using gas-liquid chromatography, thin-layer chromatography, and high pressure liquid chromatography. The methods successfully identified LSD among the compounds tested.

Study at a glance

Key finding EI/MS, when combined with other analytical techniques, is capable of differentiating LSD from any of the other compounds included in this study.

Abstract

Abstract The N-methyl-N-propyl, N-methyl-N-isopropy, N-butyl, N-isobutyl, N-sec-butyl and N-tert-butyl amides of lysergic acid were synthesized to determine the specificity of electron impact mass spectroscopy (EI/MS), when combined with other analytical techniques, for the identification of lysergic acid diethylamide (LSD). After separation of the C8 axial and equatorial isomers by preparative thin-layer chromatography, the amides were subjected to gas-liquid chromatography (GLC), thin-layer chromatography (TLC), high pressure liquid chromatography (HPLC), and EI/MS. EI/MS, when combined with other analytical techniques, is shown to be capable of differentiating LSD from any of the other compounds included in this study.

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