Mechanism of lysergic acid diethylamide interference with rabbit antibody biosynthesis
Proceedings of the National Academy of Sciences of the United States of America January 1, 1974 DOI: 10.1073/pnas.71.4.1061 via Elsevier
Summary
Lymphoid cells from hyperimmune rabbits producing antibodies to a hapten, when incubated with d-lysergic acid diethylamide (LSD), synthesized protein at a normal rate. However, the low-molecular-weight protein secreted by these cells had isoelectric points (pI) of 4.9 and 5.2, whereas immune cells not exposed to LSD secreted only 7S IgG molecules with an average pI of about 7.0. This suggests LSD alters the type of protein secreted by these immune cells.
Study at a glance
| Characteristics | Experimental study Peer reviewed |
|---|---|
| Population | Lymphoid cells from hyperimmune rabbits |
| Citations | 8 |
| Key finding | Incubation with LSD led to secretion of low-molecular-weight proteins with pI 4.9 and 5.2 instead of the typical 7S IgG with pI 7.0. |
Abstract
Lymphoid cells from hyperimmune rabbits producing antibodies to a hapten, incubated in the presence of d-lysergic acid diethylamide, continued to synthesize protein at a normal rate. Isoelectric focusing analysis of the low-molecular-weight protein secreted by the cells incubated with lysergic acid diethylamide indicated two components, with pI's of 4.9 and 5.2. Immune cells not exposed to lysergic acid diethylamide secreted only 7S IgG molecules with an average pI of approximately 7.0.