Metabolic profile determination of 25N-NBOMe in human liver microsomes by liquid chromatography-quadrupole time-of-flight mass spectrometry.
International journal of legal medicine May 1, 2019 Hyewon Seo, In Sook Kim, Young-Hoon Kim et al. 9 citations
The synthetic psychoactive substance 25N-NBOMe, a phenethylamine, was metabolized in vitro using human liver microsomes. Fourteen metabolites (M1–M14) were identified through liquid chromatography-quadrupole time-of-flight mass spectrometry. The biotransformations included hydroxylation, O-demethylation, N-dealkylation, nitro reduction, dehydrogenation, and carbonylation. The hydroxyl metabolite was the most abundant after phase I metabolism. These findings offer potential biomarkers for detecting 25N-NBOMe ingestion.