Determination of psilocybin and psilocin content in multiple Psilocybe cubensis mushroom strains using liquid chromatography - tandem mass spectrometry.
Analytica chimica acta February 1, 2024 Roman Goff, Morgan Smith, Sabrina Islam et al. 29 citations
A new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method measures psilocybin and psilocin in the mushroom species Psilocybe cubensis. Five strains—Blue Meanie, Creeper, B-Plus, Texas Yellow, and Thai Cubensis—were analyzed. The method detects psilocybin at 300 ng/g and psilocin at 30 ng/g of mushroom, with accuracy and precision errors under 6%. From most to least potent, average total psilocybin and psilocin concentrations were 1.36% (Creeper), 1.221% (Blue Meanie), 1.134% (B+), 1.103% (Texas Yellow), and 0.879% (Thai Cubensis) by weight. Results were comparable between two laboratories, and homogenizing multiple mushrooms together improved precision.