Electrochemical Simulation of 25B-NBOMe Phase I Metabolism and Metabolite Profiling by HPLC-QTOF-MS.
Molecules (Basel, Switzerland) November 18, 2025 Agata Kot-Wasik, Agnieszka Potęga, Justyna Aszyk-Woźniak et al.
Electrochemical oxidation of the psychoactive substance 25B-NBOMe in a flow cell produced key phase I metabolites—hydroxylated and N-desalkylated products along with dehydrogenated derivatives—and smaller amounts of O-desmethylated and bis-O,O-desmethylated forms. The O-desmethylated metabolite was also found in gastric contents, blood, and urine from severely intoxicated individuals. Phase II metabolites (glucuronide and sulfonate conjugates) appeared only in biological samples. The electrochemical method rapidly generated potential in vivo metabolites, partially matching authentic human samples, supporting its use as a screening tool for metabolism and toxicological risk assessment of novel psychoactive substances.