Validation of a New Sensitive Method for the Detection and Quantification of R and S-Epimers of Ergot Alkaloids in Canadian Spring Wheat Utilizing Deuterated Lysergic Acid Diethylamide as an Internal Standard
Toxins January 20, 2022 Jensen Cherewyk, Taylor Grusie-Ogilvie, Barry Blakley et al. 12 citations
A new ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method was validated to quantify six R and six S-epimers of ergot alkaloids in hard red spring wheat, using deuterated lysergic acid diethylamide (LSD-D3) as an internal standard. The method showed low limits of detection and quantification, matrix effects between 101–113%, recovery of 68.3–119.1%, and inter-day precision below 24% relative standard deviation. These validation parameters align with previous studies and reveal differences between R and S-epimers that have rarely been documented. The sensitive method can be adopted by research or diagnostic laboratories.