Determination of Lysergic Acid Diethylamide and 2-Oxo-3-Hydroxy-LSD in Blood: Validation and Comparison of Two Liquid Chromatography–Tandem Mass Spectrometry Methods
Separations September 27, 2023 Alexandra Dimitrova, Maria Grazia di Milia, Regina Rensi et al. 6 citations
Two methods for detecting LSD and its main metabolite O-H-LSD in blood were validated using LC-MS/MS. Method #1 upgraded an existing multi-compound screening procedure, while Method #2 was tailored specifically for these substances. Both methods showed good linearity and sensitivity, with limits of quantification as low as 0.025 ng/mL for LSD and 0.0125 ng/mL for O-H-LSD. Bias and precision met acceptance criteria; recoveries exceeded 83% except for O-H-LSD with Method #1. Applied successfully to two real cases, Method #1 is suitable for screening, and Method #2 provides a sensitive, reliable tool for confirmation.