Administration of Δ9-Tetrahydrocannabinol (THC) in Adolescent and Adult Mice.
Journal of visualized experiments : JoVE August 1, 2025 Kwang-Mook Jung, Alex Mabou Tagne, Heidi C Avalos et al.
THC, the intoxicating component of cannabis, activates CB1 and CB2 cannabinoid receptors in the brain and body, affecting numerous physiological processes. The endocannabinoid system's functions change across the lifespan, with adolescence being a period of particular sensitivity. This article describes a protocol for preparing and administering THC to adolescent and young adult mice of both sexes via intraperitoneal injection at doses of 1, 5, and 10 mg/kg. The protocol illustrates THC's well-known effects: catalepsy, reduced locomotor activity, and suppressed nociception. Procedures for legally obtaining and storing THC in compliance with U.S. regulations are also outlined.