Determination of ibogaine in plasma by gas chromatography--chemical ionization mass spectrometry.
F R Ley, A R Jeffcoat, B F Thomas
Journal of chromatography. A February 2, 1996 DOI: 10.1016/0021-9673(95)00827-6 via PubMed
Summary
AI-generated from the abstractA new analytical method using gas chromatography-mass spectrometry with a stable-isotope internal standard can measure ibogaine levels in one milliliter of plasma. The technique is selective and sensitive, addressing a gap in quantitative methods that has limited pharmacokinetic and metabolism studies of ibogaine, a naturally occurring alkaloid being considered as a treatment for drug dependence.
Study at a glance
| Characteristics | Method development Peer reviewed |
|---|---|
| Citations | 18 |
| Key finding | A gas chromatography-mass spectrometry method with a synthesized [13C2H3]ibogaine internal standard provides a selective and sensitive way to quantify ibogaine in plasma. |
Abstract
Ibogaine is naturally occurring indole alkaloid that is currently being considered as a treatment medication for drug dependence. Although there have been a variety of investigations regarding the mechanisms of action and pharmacology of ibogaine, relatively little has been reported regarding quantitative methods. Because of the paucity of analytical methodologies, studies involving the pharmacokinetics and metabolism of ibogaine have also been limited. A method is described for the determination of ibogaine levels in plasma by gas chromatography -- methane chemical ionization mass spectrometry. [13C2H3]Ibogaine was synthesized and used as an internal standard to control for recovery during sample preparation. The assay requires one ml of plasma and is shown to be a selective and sensitive means of ibogaine quantitation.