LC/MS/MS analysis of the endogenous dimethyltryptamine hallucinogens, their precursors, and major metabolites in rat pineal gland microdialysate.
Steven A Barker, Jimo Borjigin, Izabela Lomnicka, Rick Strassman
Biomedical chromatography : BMC December 1, 2013 DOI: 10.1002/bmc.2981 via PubMed
Summary
AI-generated from the abstractA qualitative liquid chromatography-tandem mass spectrometry method was developed to simultaneously analyze three known N,N-dimethyltryptamine endogenous hallucinogens, their precursors and metabolites, as well as melatonin and its metabolic precursors. The method was characterized using artificial cerebrospinal fluid and applied to rat brain pineal gland microdialysate. It allows direct injection of 23 chemically diverse compounds plus a deuterated internal standard without dilution or extraction. The approach is simple, sensitive, specific, and uses stringent MS confirmatory criteria including exact mass measurements. For the first time, N,N-dimethyltryptamine was detected in pineal gland microdialysate from the rat.
Study at a glance
| Characteristics | Qualitative method development and application Peer reviewed |
|---|---|
| Population | Rat brain pineal gland microdialysate |
| Keywords | Lc/ms/ms N,N-Dimethyltryptamines Microdialysis Pineal gland Rat brain |
| Citations | 73 |
| Key finding | N,N-dimethyltryptamine was detected for the first time in pineal gland microdialysate obtained from the rat. |
Abstract
We report a qualitative liquid chromatography-tandem mass spectrometry (LC/MS/MS) method for the simultaneous analysis of the three known N,N-dimethyltryptamine endogenous hallucinogens, their precursors and metabolites, as well as melatonin and its metabolic precursors. The method was characterized using artificial cerebrospinal fluid (aCSF) as the matrix and was subsequently applied to the analysis of rat brain pineal gland-aCSF microdialysate. The method describes the simultaneous analysis of 23 chemically diverse compounds plus a deuterated internal standard by direct injection, requiring no dilution or extraction of the samples. The results demonstrate that this is a simple, sensitive, specific and direct approach to the qualitative analysis of these compounds in this matrix. The protocol also employs stringent MS confirmatory criteria for the detection and confirmation of the compounds examined, including exact mass measurements. The excellent limits of detection and broad scope make it a valuable research tool for examining the endogenous hallucinogen pathways in the central nervous system. We report here, for the first time, the presence of N,N-dimethyltryptamine in pineal gland microdialysate obtained from the rat.