Extraction and analysis of indole derivatives from fungal biomass
Journal of Basic Microbiology January 1, 1994 DOI: 10.1002/jobm.3620340104 via OpenAlex
Summary
AI-generated from the abstractThe highest concentrations of psilocybin and baeocystin in six species of higher fungi were obtained by extraction with pure methanol. Psilocin content appeared higher when using aqueous alcohol solutions, but this was an artifact of enzymatic breakdown of psilocybin. Extraction with dilute acetic acid outperformed water-containing alcohols. Methanol extraction remains the safest one-step method for obtaining genuine alkaloids from fungal biomass.
Study at a glance
| Characteristics | Comparative extraction study Peer reviewed |
|---|---|
| Population | Six species from four genera of higher fungi |
| Intervention | dilute acetic acid |
| Keywords | Indole test Biomass ecology Extraction chemistry Environmental science Environmental chemistry |
| Citations | 56 |
| Key finding | Methanol extraction yields the highest concentrations of psilocybin and baeocystin and is the safest method for obtaining genuine alkaloids from fungal biomass. |
Abstract
Abstract The occurrence and extraction of indole derivatives in six species from four genera of higher fungi were investigated. By using pure methanol for extraction of the mushrooms analysis revealed the highest concentrations of psilocybin and baeocystin. The psilocin content of the species was higher by using aqueous solutions of alcohols than with methanol alone but was an artificial phenomenon caused by enzymatic destruction of psilocybin. The extraction with dilute acetic acid yielded better results than with the water containing alcohols. The simple one‐step procedure with methanol for the quantitative extraction is still the safest method to obtain the genuine alkaloids from fungal biomass.