A draft reference assembly of the Psilocybe cubensis genome
Kevin Mckernan, Liam T. Kane, Seth Crawford, Chen-Shan Chin, Aaron Trippe, Stephen Mclaughlin
F1000Research June 15, 2021 DOI: 10.12688/f1000research.51613.2 via OpenAlex
Summary
AI-generated from the abstractThe genome of the psychoactive Psilocybe cubensis mushroom was assembled using high-fidelity single molecule sequencing. The genome is 46.6 megabases in size, has a guanine-cytosine content of 46%, and is arranged in 32 contigs with an N50 of 3.3 megabases. Completeness assessment shows 97.6% of conserved genes are present, with 1.2% duplicated. The psilocybin synthesis cluster is contained within a single 3.2 megabase contig. The genome data are publicly available through NCBI BioProject under accessions PRJNA687911 and PRJNA700437.
Study at a glance
| Characteristics | Genome assembly Peer reviewed |
|---|---|
| Population | Psilocybe cubensis mushroom |
| Keywords | Contig Genome Computational biology Genetics Alkaloids |
| Citations | 10 |
| Key finding | The genome of Psilocybe cubensis was assembled into 32 contigs, with the psilocybin synthesis cluster located on a single 3.2 megabase contig. |
Abstract
We describe the use of high-fidelity single molecule sequencing to assemble the genome of the psychoactive Psilocybe cubensis mushroom. The genome is 46.6Mb, 46% GC, and in 32 contigs with an N50 of 3.3Mb. The BUSCO completeness scores are 97.6% with 1.2% duplicates. The Psilocybin synthesis cluster exists in a single 3.2Mb contig. The dataset is available from NCBI BioProject with accessions PRJNA687911 and PRJNA700437.