Determination of LSD in Urine by Capillary Column Gas Chromatography and Electron Impact Mass Spectrometry
Paula Francom, David M. Andrenyak, Hyun Kyoon Lim, Robert R. Bridges, R. L. Foltz, R. T. Jones
Journal of Analytical Toxicology January 1, 1988 DOI: 10.1093/jat/12.1.1 via OpenAlex
Summary
AI-generated from the abstractA method detects LSD in urine at concentrations as low as 0.5 ng/mL. After adding a deuterium-labeled internal standard, LSD is extracted from urine at pH 8 using n-butyl chloride, then converted to a trimethylsilyl derivative and measured by gas chromatography–mass spectrometry with selected ion monitoring. The procedure tracked LSD concentrations in urine for eight hours after two volunteers each took 70.5 micrograms of LSD orally. Results are compared with those from radioimmunoassay and high-performance liquid chromatography. The report also includes data on LSD stability in urine.
Study at a glance
| Characteristics | Method development and validation Peer reviewed |
|---|---|
| Sample size | 2 |
| Population | Human volunteers |
| Keywords | Urine Chemistry Gas chromatography–mass spectrometry Capillary gas chromatography Electron ionization |
| Citations | 55 |
| Key finding | LSD can be quantified in urine down to 0.5 ng/mL using gas chromatography–mass spectrometry after extraction and derivatization. |
Abstract
A procedure for the determination of LSD (lysergic acid diethylamide) in urine at concentrations as low as 0.5 ng/ml is presented. After addition of deuterium-labeled LSD as the internal standard, a rapid n-butyl chloride extraction of LSD from urine at pH 8 is followed by formation of the trimethylsilyl (TMS) derivative by treatment with N,O-bis(trimethylsilyl)trifluoroacetamide. The TMS derivative of LSD is identified and quantified by selected ion monitoring with a fused-silica capillary column and electron impact ionization. The procedure was used to monitor LSD concentrations in urine for eight hours following oral administration of 70.5 micrograms of LSD to two human volunteers. Concentrations of LSD determined by the assay are compared with concentrations determined by two other methods of analysis, a radioimmunoassay and a high-performance liquid chromatographic (HPLC) assay. Data concerning the stability of LSD in urine are also presented.