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Gas chromatographic/mass spectrometric determination of lysergic acid diethylamide (LSD) in serum samples

Frank Mußhoff, T. Daldrup

Forensic Science International August 1, 1997 DOI: 10.1016/s0379-0738(97)00063-7 via OpenAlex

Summary

AI-generated from the abstract

A method to detect and measure LSD in blood serum uses liquid-liquid extraction followed by gas chromatography-mass spectrometry of a trimethylsilyl derivative. Tests on spiked samples recovered 76% of the LSD, with a coefficient of variation of 9.3%. The method is linear across 0.1–10 ng/ml. The work also presents evidence of LSD's sensitivity to light and includes case examples.

Study at a glance

Characteristics Method development and validation Peer reviewed
Topics LSD
Keywords Gas chromatography–mass spectrometry Coefficient of variation Extraction chemistry Detection limit
Citations 29
Key finding A gas chromatography-mass spectrometry method can reliably detect and quantify LSD in serum with 76% recovery and linearity from 0.1 to 10 ng/ml.

Abstract

A sensitive method for the detection and quantification of lysergic acid diethylamide (LSD) in serum samples is described. After liquid-liquid extraction the trimethylsilyl derivative of LSD is detected by gas chromatography-mass spectrometry. Experiments with spiked samples resulted in a recovery of 76%, the coefficient of variation was 9.3%. Excellent linearity was obtained over the range 0.1-10 ng ml-1. Additionally experiments demonstrating the light sensitivity of LSD are presented together with casuistics.

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