Lysergic Acid Diethylamide (LSD) Binding to Deoxyribonucleic Acid (DNA)
Experimental Biology and Medicine August 1, 1968 DOI: 10.3181/00379727-128-33303 via OpenAlex
Summary
AI-generated from the abstractAdding DNA, but not RNA, to solutions of lysergic acid diethylamide (LSD) lowered the drug's absorption and fluorescence spectra. The binding affinity between LSD and DNA had a dissociation constant (Kd) of 5 × 10⁻⁴ M, with each nucleotide residue serving as a potential binding site. Binding did not occur at pH 3, where DNA is nonhelical, and was reduced by heating the DNA beforehand or by adding magnesium chloride at 3.7 × 10⁻³ M.
Study at a glance
| Characteristics | Experimental study Peer reviewed |
|---|---|
| Topics | LSD |
| Keywords | Dna Fluorescence Nucleotide Binding site |
| Citations | 23 |
| Key finding | LSD binds to DNA with a Kd of 5 × 10⁻⁴ M, and binding depends on DNA's helical structure and is inhibited by heat or magnesium chloride. |
Abstract
SummaryAddition of DNA (but not RNA) to solutions of lysergic acid diethylamide resulted in a depression of the absorption and fluorescence spectra of the drug. The Kd for D-LSD: DNA binding, calculated from spectral changes, was 5 × 10-4 M, and each nucleotide residue was a potential binding site. Binding did not occur at pH 3 (where DNA is nonhelical), and was decreased by prior heating of the DNA or by adding MgCl2 at a concentration of 3.7 × 10-3M.