Determination of synthetic hallucinogens in oral fluids by microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry.
Evan Lesne, Miguel Muñoz-Bartual, Francesc A Esteve-Turrillas
Analytical and bioanalytical chemistry July 1, 2023 DOI: 10.1007/s00216-023-04751-2 via PubMed
Summary
AI-generated from the abstractA fast and simple method using microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry was developed to simultaneously quantify 28 synthetic hallucinogens in oral fluids, including LSD and substances from NBOMe, NBOH, NBF, 2C, and substituted amphetamine classes. Optimal extraction used C18 sorbent, 100 μL sample at pH 7 loaded in 3 cycles, washing with water, and eluting with 50 μL methanol in 1 cycle, yielding quantitative recoveries (80–129%) and no significant matrix effects. Limits of detection ranged from 0.09 to 1.22 μg L⁻¹. Precision was high, with relative standard deviations below 9%. The method is appropriate for simple, sensitive determination of NBOMe derivatives and other synthetic hallucinogens in oral fluid samples.
Study at a glance
| Characteristics | Method development and validation Peer reviewed |
|---|---|
| Population | Spiked oral fluid samples |
| Keywords | Nbomes New psychoactive substances Oral fluids Drug testing Forensic toxicology |
| Citations | 14 |
| Key finding | A MEPS-LC-MS/MS method using C18 sorbent with optimized conditions achieved quantitative recoveries (80–129%), low detection limits (0.09–1.22 μg L⁻¹), and high precision (RSD <9%) for 28 synthetic hallucinogens in oral fluid. |
Abstract
A fast and simple procedure based on microextraction by packed sorbent (MEPS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed for the simultaneous quantification of 28 synthetic hallucinogens in oral fluids, including lysergic acid diethylamide and substances from NBOMe, NBOH, NBF, 2C, and substituted amphetamine categories. Extraction conditions such as type of sorbent, sample pH, number of charge/discharge cycles, and elution volume were studied. Hallucinogenic compounds were extracted from oral fluid samples using C18 MEPS, loading with 100 μL sample (adjusted to pH 7) in 3 cycles, washing with 100 μL deionized water, and eluting with 50 μL methanol in 1 cycle, giving quantitative recoveries and no significant matrix effects. Limits of detection from 0.09 to 1.22 μg L-1; recoveries from 80 to 129% performed in spiked oral fluid samples at 20, 50, and 100 μg L-1; and high precision with relative standard deviations lower than 9% were obtained. The proposed methodology was demonstrated to be appropriate for the simple and sensitive determination of NBOMe derivates and other synthetic hallucinogenic substances in oral fluid samples.