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In vivometabolism ofα-methyltryptamine in rats: Identification of urinary metabolites

Tatsuyuki Kanamori, Kenji Kuwayama, Kenji Tsujikawa, Hajime Miyaguchi, Yuko Iwata, Hiroyuki Inoue

Xenobiotica November 3, 2008 DOI: 10.1080/00498250802491654 via OpenAlex

Summary

AI-generated from the abstract

Alpha-methyltryptamine (AMT), a psychoactive tryptamine analogue, is metabolized in rats into at least four distinct compounds. After oral administration of 10 mg/kg to male Wistar rats, urine collected over 24 hours was enzymatically hydrolyzed, extracted, and analyzed by gas chromatography/mass spectrometry. The detected metabolites were 2-oxo-AMT, 6-hydroxy-AMT, 7-hydroxy-AMT, and 1'-hydroxy-AMT. These findings identify specific metabolic pathways for AMT, which may inform understanding of its pharmacological effects and duration of action.

Study at a glance

Characteristics In vivo animal study Peer reviewed
Population Male Wistar rats
Keywords Urine Tryptamine In vivo Metabolism Metabolite
Citations 11
Key finding AMT is metabolized in rats to 2-oxo-AMT, 6-hydroxy-AMT, 7-hydroxy-AMT, and 1'-hydroxy-AMT.

Abstract

1. The in vivo metabolism of alpha-methyltryptamine (AMT), a psychoactive tryptamine analogue, was studied in rats. 2. Male Wistar rats were administered 10 mg kg(-1) AMT orally and 24-h urine fractions were collected. After enzymatic hydrolysis of the urine sample, the metabolites were extracted by liquid-liquid extraction and analysed by gas chromatography/mass spectrometry (GC/MS). 3. 2-Oxo-AMT, 6-hydroxy-AMT, 7-hydroxy-AMT and 1'-hydroxy-AMT were detected as metabolites of AMT.

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