GLC-mass Spectral Determination of Mescaline in Plasma of Rabbits after Intravenous Injection

A gas-liquid chromatography–mass spectrometry method using a deuterated internal standard accurately measures mescaline concentrations in rabbit plasma. After intravenous administration, mescaline and the internal standard are extracted with benzene, derivatized with trifluoroacetic acid anhydride, and separated on a 2.5% QF-1 column with mass fragmentographic detection. The detection limit is 5 ng/ml of plasma, and the relative standard deviation is about 5%. The method's main advantage is combining the specificity of gas-liquid chromatography retention time and mass spectral fragmentation with the sensitivity of mass fragmentographic detection.

Study at a glance

Abstract

A GLC-mass spectral analysis with a deuterated internal standard was developed to measure plasma mescaline concentrations after intravenous administration to rabbits. The drug and the internal standard were extracted with benzene, derivatized with trifluoroacetic acid anhydride, and chromatographed on 2.5% QF-1 with mass fragmentographic detection. The detection limit is 5 ng/ml of plasma. The relative standard deviation was approximately 5%. The main advantage of this method is that it combines the specificity of the GLC retention time and mass spectral fragmentation pattern with the sensitivity of the mass fragmentographic detection.