Binding of Mescaline with Subcellular Fractions upon Incubation of Brain Cortex Slices with [14C] Mescaline
R. K. Datta, W Antopol Dagger, J. J. Ghosh
Pharmacology January 1, 1977 DOI: 10.1159/000136700 via OpenAlex
Summary
AI-generated from the abstractWhen brain cortex slices were incubated with glucose, about 65% of radioactive mescaline entered the slices. Roughly one-third of this radioactivity became bound to subcellular components including nuclei, mitochondria, microsomes, and ribosomes. Dialysis did not substantially remove the bound radioactivity. The uptake into slices and binding to subcellular fractions increased over time but were reduced by potassium cyanide, absence of glucose, or heating to 80°C for one minute.
Study at a glance
| Characteristics | In vitro experimental study Peer reviewed |
|---|---|
| Population | Brain cortex slices |
| Topics | Mescaline |
| Keywords | Incubation Cortex anatomy Microsome Methemoglobin |
| Citations | 1 |
| Key finding | Mescaline permeates brain cortex slices and binds to subcellular fractions in a time-dependent, energy-requiring process. |
Abstract
Incubation of brain cortex slices in the presence of glucose resulted in the permeation of about 65% of [14C] mescaline into slices. Of this, about one-third radioactivity was bound with nuclei, mitochondria, microsomes, and ribosomes. Dialysis of subcellular fractions did not markedly reduce the amounts of radioactivity bound to the fractions. The permeation into slices and the binding of mescaline to subcellular fractions were fairly time-dependent, but were inhibited by the presence of potassium cyanide, or by the absence of glucose and by heating to 80 degrees C for 1 min.