Increased brain VDAC1 protein levels correlate with behavioural performance in a ketamine murine model of schizophrenia and are attenuated by the mGluR2 modulator JNJ-46356479.

In a mouse model of schizophrenia induced by postnatal ketamine exposure, the mitochondrial protein VDAC1 was elevated in the prefrontal cortex, and this increase was partially reversed by treatment with JNJ-46356479, a modulator of metabotropic glutamate receptors. Higher VDAC1 levels correlated with a greater Bax/Bcl-2 ratio—indicating apoptotic imbalance—and with worse performance on tests of memory and social behavior. VDAC1 may serve as a marker of apoptotic dysfunction in schizophrenia, and glutamatergic modulation could represent a therapeutic strategy targeting mitochondrial pathology.

Study at a glance

Abstract

Schizophrenia (SZ) is a neurodevelopmental disorder involving excitatory/inhibitory imbalance and apoptotic dysregulation. VDAC1, a voltage-dependent anion channel protein located in the mitochondrial outer membrane, plays a pivotal role in mitochondria-mediated apoptosis. However, its involvement in SZ remains underexplored. This study aimed to investigate VDAC1 protein levels in the prefrontal cortex (PFC) and hippocampus (HPC) of a postnatal ketamine-exposed mouse model of SZ, and to assess the effects of treatment with the metabotropic GLU receptor 2 (mGluR2) positive allosteric modulator JNJ-46356479 (JNJ). We also examined associations between VDAC1 expression, behavioural performance, and other apoptosis-related markers. Male and Female C57BL/6 J mice were exposed to ketamine or saline on postnatal days (PND) 7, 9, and 11, and treated in adulthood with JNJ or vehicle. VDAC1 protein levels in the PFC and HPC were quantified by western blot. Previously acquired behavioural data and levels of apoptotic markers (Bax, Bcl-2, caspase-3) from the same animals were analysed in relation to VDAC1 expression. VDAC1 levels were significantly increased in the PFC of ketamine-exposed mice and partially normalised by JNJ treatment. VDAC1 levels in the PFC and HPC were positively correlated. Higher VDAC1 levels were associated with an increased Bax/Bcl-2 ratio and poorer cognitive and social performance, particularly in the Y-Maze and the Five-Trial Social Memory Tests. Our findings support VDAC1 as a potential molecular marker of apoptotic imbalance in SZ. JNJ treatment may exert neuroprotective effects by modulating VDAC1 levels, suggesting that glutamatergic modulation could offer novel therapeutic avenues targeting mitochondrial dysfunction in SZ.