Automated Detection of Psychedelic-Induced Head-Twitch Response in Mice
Alaina M. Jaster, Javier González-Maeso
Schizophrenia January 1, 2023 DOI: 10.1007/978-1-0716-3307-6_6 via Springer Nature
Summary
AI-generated from the abstractThe head-twitch response (HTR) in rodents is a behavioral marker of serotonin 2A receptor activation caused by psychedelics. This work describes a method using a magnetic ear tag reporter with automated quantification and biphasic detection to measure HTR in mice given the psychedelic DOI. The approach can help study molecular mechanisms of psychosis and identify signaling processes relevant to antipsychotic and psychedelic compounds.
Study at a glance
| Characteristics | Methodological description Peer reviewed |
|---|---|
| Population | Mice |
| Interventions | 1-(2 5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) |
| Keywords | Head-twitch response htr Behavioral model Psychosis Psychedelics Hallucinogen |
| Key finding | A magnetic ear tag reporter with automated quantification and biphasic detection can identify head-twitch response in mice treated with DOI. |
Abstract
Head-twitch response (HTR) allows for the detection and classification of behavior associated with serotonin 2A receptor (5-HT_2AR) activation upon psychedelic administration in rodent models. This activation and functional output can be utilized to provide insights into molecular mechanisms associated with psychosis and to identify signaling processes related to existing and novel antipsychotic and psychedelic compounds. Here we describe the use of a magnetic ear tag reporter coupled with automated quantification and biphasic detection to identify HTR in mice treated with the classical psychedelic 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI).