[DNA and chemical analyses of commercial fly agaric-related products].
Takuro Maruyama, Nobuo Kawahara, Toshimitsu Fukiharu, Kazumasa Yokoyama, Yukiko Makino, Yukihiro Goda
Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan April 1, 2005 DOI: 10.3358/shokueishi.46.49 via PubMed
Summary
AI-generated from the abstractSince 2002, psilocybin-containing fungi have been regulated in Japan, but fly agaric-related products are now entering the Japanese market via the internet. DNA analysis of these products suggested they originate from Amanita muscaria or A. muscaria var. persicina, and they were classified into three strains based on nucleotide sequences. Chemical analysis detected harmine derivatives and/or tryptamine derivatives in some products, and the matK gene of Peganum harmala was found in all harmine-containing samples, indicating the presence of additional psychoactive plants.
Study at a glance
| Characteristics | Observational study Peer reviewed |
|---|---|
| Population | Fly agaric-related products available via the internet in Japan |
| Key finding | Fly agaric products sold in Japan originate from A. muscaria or A. muscaria var. persicina, and some contain harmine derivatives from Peganum harmala. |
Abstract
Since June 6, 2002, psilocin and psilocybin-containing fungi (commonly called "magic mushrooms") have been regulated by the Narcotics and Psychotropics Control Law in Japan. However, various fly agaric-related products are now entering the Japanese market via the internet. In this study, fly agaric-related products available in this way were investigated for raw materials by DNA analysis and for additives by chemical analysis. Nucleotide sequence analysis of the mitochondrial 12S rDNA region suggested that these fly agaric-related products originate from A. muscaria or A. muscaria var. persicina. Furthermore, they were classified into three strains based on the ITS2-LSU nucleotide sequence. Harmine derivatives and/or tryptamine derivatives were detected in some of these products by LC/MS analysis. In accordance with this, the matK gene of Peganum harmala was found in all of the harmine derivative-containing samples.