Analysis of Salvinorin A in plants, water, and urine using solid-phase microextraction-comprehensive two-dimensional gas chromatography-time of flight mass spectrometry.
Journal of chromatography. A – February 24, 2012
Source: PubMed
Summary
Detecting the hallucinogen Salvinorin A in plants, water, and urine is now significantly more efficient. A new method utilizing solid-phase microextraction (SPME) alongside advanced mass spectrometry proved superior. While initial plant analysis revealed lower-than-expected Salvinorin A levels, SPME demonstrated high effectiveness for water and urine samples, achieving excellent sensitivity (5 ng/mL detection) and precision. This robust analytical technique excels at isolating target compounds, providing a powerful tool for identifying and quantifying Salvinorin A.
Abstract
Salvinorin A, a psychoactive hallucinogen, and related compounds, were analyzed in plants, water, and urine using liquid-liquid extraction (LLE), solid-phase microextraction (SPME) and comprehensive two-dimensional gas chromatography-time of flight mass spectrometry (GC×GC-ToFMS). A semi-qualitative study of the extraction of Salvinorin A and analogs from Salvia divinorum plants by LLE showed ppb levels of Salvinorin A and several analogs in the leaves and stems of S. divinorum plants, much lower than expected. Quantitative analysis of Salvinorin A spiked into water and urine showed much better figures of merit for SPME than LLE, with limit of detection of about 5 ng/mL, linear range from 8 to 500 ng/mL and precision about ±10% for the SPME-based analyses using external standard quantitation. GC×GC-ToFMS was especially effective in separating the peaks of interest from matrix and chromatographic interferences.