Simultaneous determination of thirteen plant alkaloids in a human specimen by SPE and HPLC.
Journal of separation science – July 01, 2008
Source: PubMed
Summary
Thirteen common plant toxins, from nicotine to strychnine, can now be precisely identified and measured in human samples with unprecedented ease. A new, robust analytical method employing advanced extraction and chromatography offers highly accurate and sensitive detection. This breakthrough is invaluable for quickly diagnosing intoxications, monitoring drug abuse, and ensuring compliance in both forensic and clinical applications.
Abstract
A new screening method for the simultaneous determination of thirteen plant alkaloids (aconitine, anabasine, atropine, brucine, colchicine, cotinine, cytisine, harmine, ibogaine, nicotine, scopolamine, strychnine, yohimbine) in a human specimen was developed based on solid-phase extraction and reversed-phase liquid chromatography with photodiode array detection. The validated method enables selective identification as well as accurate and sensitive quantification. The analysis of forensic and clinical samples emphasizes the applicability for intoxications and drug abuse, as well as for compliance control.