The bioactivity of 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) and its detection in rat urine by capillary electrophoresis combined with an on-line sample concentration technique.
Yu-Chih Chiu, Shiu-Huey Chou, Ju-Tsung Liu, Cheng-Huang Lin
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences November 25, 2004 DOI: 10.1016/j.jchromb.2004.08.021 via PubMed
Summary
Oral administration of 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) to mice decreased nitric oxide (NO) production by T and B lymphocytes in the spleen and T cells in the thymus, suggesting the drug may weaken immune defense functions. In rats, the parent drug was detected in urine using capillary electrophoresis with UV absorbance. Without sample concentration, detection limits were 4.5 and 5.0 μg/mL; with stacking and sweeping-micellar electrokinetic chromatography, limits improved to 19.2 and 9.1 ng/mL. After intra-peritoneal injection of 20 μg/g body weight in three male rats, 2.9 μg/mL and 0.25 μg/mL of 2C-T-2 were found in first- and second-day urine fractions.
Study at a glance
| Characteristics | Experimental study Peer reviewed |
|---|---|
| Population | Mice and rats |
| Keywords | Analytical chemistry Capillary electrophoresis 2c-t-2 Toxicology Biomonitoring |
| Citations | 18 |
| Key finding | 2C-T-2 reduced NO production in mouse spleen and thymus lymphocytes, and the drug was detectable in rat urine at concentrations down to nanogram per milliliter levels using on-line sample concentration techniques. |
Abstract
The bioactivity of 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) on nitric oxide (NO) production and the proliferation of spleen and thymus lymphocytes to mitogen stimulation in mice are reported for the first time. NO production by T and B lymphocytes in spleen and T cells in the thymus of mice decreased after the oral administration of 2C-T-2. This indicates that 2C-T-2 intake may perturb both neural and immune activity since a decrease in NO production is indicative of a weakened defense function. 2C-T-2 (the parent drug) in rat urine samples was detected by means of capillary electrophoresis/UV absorbance combined with an on-line sample concentration technique. When the CZE and MEKC modes were employed, the detection limit was found to be 4.5 and 5.0 microg/mL (at a 92.1% confidence level); whereas when on-line sample concentration methods, including stacking and sweeping-micellar electrokinetic chromatography were used, the detection limits were improved to 19.2 and 9.1 ng/mL, respectively. In an analysis of some actual samples from animal experiments, three male rats were administered 20 microg/g of body weight of 2C-T-2 by intra-peritoneal injection. The first- and second-day urine fractions were collected after the administration, for use in the analysis. As a result, 2.9 microg/mL and 0.25 microg/mL of 2C-T-2, respectively, were detected after ingestion of the doses.