The bioactivity of 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) and its detection in rat urine by capillary electrophoresis combined with an on-line sample concentration technique.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences November 25, 2004 Yu-Chih Chiu, Shiu-Huey Chou, Ju-Tsung Liu et al. 18 citations
Oral administration of 2,5-dimethoxy-4-ethylthiophenethylamine (2C-T-2) to mice decreased nitric oxide (NO) production by T and B lymphocytes in the spleen and T cells in the thymus, suggesting the drug may weaken immune defense functions. In rats, the parent drug was detected in urine using capillary electrophoresis with UV absorbance. Without sample concentration, detection limits were 4.5 and 5.0 μg/mL; with stacking and sweeping-micellar electrokinetic chromatography, limits improved to 19.2 and 9.1 ng/mL. After intra-peritoneal injection of 20 μg/g body weight in three male rats, 2.9 μg/mL and 0.25 μg/mL of 2C-T-2 were found in first- and second-day urine fractions.