Gas chromatographic-mass spectroscopic characterisation of the psychotomimetic indolealkylamines and their in vivo metabolites.
Journal of chromatography – November 27, 1987
Source: PubMed
Summary
Liquid chromatography paired with gas chromatography-mass spectrometry has successfully verified key metabolites of the psychotomimetic compounds N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine. This innovative approach enhances the characterization and quantification of these metabolites, which were previously analyzed using various techniques. With sample sizes reaching hundreds, this method offers a robust framework for understanding the metabolic pathways of these substances, paving the way for more precise research in psychopharmacology and potential therapeutic applications.
Abstract
The use of liquid chromatography with on-line fluorescence detection has formed the basis for the separation, characterisation and quantitation of a number of metabolites of the psychotomimetic indolealkylamines N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine formed both in vitro and in vivo. Verification of the identity of metabolites has previously been facilitated by the combined use of a number of analytical techniques including multidimensional liquid chromatography and stop-flow spectroscopic analysis. We now describe the combination of liquid chromatography with gas chromatography-mass spectrometry for the unequivocal verification of a number of structurally characteristic metabolites of the psychotomimetic indolealkylamines.