Study of metabolism of psychotomimetic indolealkylamines by rat tissue extracts using liquid chromatography.
Biochemical pharmacology – May 01, 1987
Source: PubMed
Summary
A comprehensive analysis of the metabolites from the psychotomimetic compounds N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine revealed significant metabolic pathways in rat liver, kidney, and brain tissues. Key processes included oxidative deamination, N-demethylation, O-demethylation, and N-oxidation. In this study, various liquid chromatographic techniques were employed to separate and characterize these metabolites, enhancing understanding of their biochemical processing. Notably, the quantitative impact of each metabolic route was evaluated using N,N-dimethyltryptamine as a substrate, providing valuable insights into its metabolism.
Abstract
The use of a series of liquid chromatographic techniques involving cation-exchange, reverse-phase and normal-phase chromatography has permitted the separation and characterisation of a number of metabolites of the psychotomimetic indolealkylamines N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine which were isolated following incubation of these compounds with rat tissue extracts. In liver, kidney and brain tissue extracts the routes of metabolism identified included oxidative deamination, N-demethylation, O-demethylation and N-oxidation. The quantitative significance of individual routes of metabolism in these tissues was assessed using N,N-dimethyltryptamine as a substrate.