Determination and Confirmation of Psychedelic and Psychoactive Compounds in Botanical Materials by UPLC-ESI-MS/MS: Single-Laboratory Validation.

J AOAC Int  – January 01, 2026

Source: PubMed Central

Summary

Identifying psychedelic compounds in botanical materials is now highly reliable. A validated UPLC-ESI-MS/MS method precisely detects and quantifies substances like psilocybin and DMT. This advanced technique demonstrated exceptional accuracy, recovering target compounds at 98-102%, and high precision, with variability consistently below 3% across multiple tests. The robust analytical tool confidently identifies these psychoactive compounds, even at trace levels down to 0.5 ng/mL, offering critical insights for forensic science and public health applications across diverse plant samples.

Abstract

Determination and Confirmation of Psychedelic and Psychoactive Compounds in Botanical Materials by UPLC-ESI-MS/MS: Single-Laboratory Validation.

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