Measurement of Lysergic Acid Diethylamide (LSD) in Human Plasma by Gas Chromatography/Negative Ion Chemical Ionization Mass Spectrometry*
Journal of Analytical Toxicology – May 01, 1990
Source: OpenAlex
Summary
A novel method allows for precise measurement of lysergic acid diethylamide (LSD) in plasma, achieving linear responses from 0.1 to 3.0 ng/mL. Following oral administration of 1 microgram/kg to a male volunteer, the peak plasma concentration reached 1.9 ng/mL three hours later, with an apparent half-life of 5.1 hours. Utilizing advanced techniques like gas chromatography-mass spectrometry and selected ion monitoring, this approach enhances understanding of LSD's pharmacokinetics, contributing valuable insights into its effects on the body.
Abstract
A previously reported procedure for quantification of LSD in urine was modified to permit measurement of the drug in plasma. After addition of deuterium-labelled LSD, the plasma is extracted and the extract is treated with trifluoroacetylimidazole to convert the LSD to its N-trifluoroacetyl derivative. The derivatized LSD is analyzed by capillary column gas chromatography/negative ion chemical ionization. Plasma fortified with known concentrations of LSD gave linear responses from 0.1 to 3.0 ng/mL with this assay. The method was used to determine pharmacokinetic parameters for LSD after oral administration (1 microgram/kg) to a male volunteer. The apparent plasma half-life was determined to be 5.1 h. The peak plasma concentration of 1.9 ng/mL occurred 3 h after administration.