The Quantitation of 2-Oxo-3-hydroxy Lysergic Acid Diethylamide (O-H-LSD)in Human Urine Specimens, a Metabolite of LSD: Comparative Analysis Using Liquid Chromatography-Selected Ion Monitoring Mass Spectrometry and Liquid Chromatography-Ion Trap Mass Spectrometry
Journal of Analytical Toxicology – April 01, 2000
Source: OpenAlex
Summary
The detection of 2-oxo-3-hydroxy lysergic acid diethylamide (O-H-LSD), a key LSD metabolite, shows promising advancements in forensic analysis. In a study involving 68 human urine samples, O-H-LSD concentrations were found to be approximately 16 times higher than LSD levels. Both liquid chromatography-mass spectrometry methods demonstrated linear calibration curves over a range of 0-8,000 pg/mL, with detection limits at 400 pg/mL. These efficient techniques could enhance LSD detection windows and may influence drug-testing protocols in workplaces.
Abstract
This paper compares the potential forensic application of two sensitive and rapid procedures (liquid chromatography-mass spectrometry and liquid chromatography-ion trap mass spectrometry) for the detection and quantitation of 2-oxo-3-hydroxy lysergic acid diethylamide (O-H-LSD) a major LSD metabolite. O-H-LSD calibration curves for both procedures were linear over the concentration range 0-8,000 pg/mL with correlation coefficients (r2) greater than 0.99. The observed limit of detection (LOD) and limit of quantitation (LOQ) for O-H-LSD in both procedures was 400 pg/mL. Sixty-eight human urine specimens that had previously been found to contain LSD by gas chromatography-mass spectrometry were reanalyzed by both procedures for LSD and O-H-LSD. These specimens contained a mean concentration of O-H-LSD approximately 16 times higher than the LSD concentration. Because both LC methods produce similar results, either procedure can be readily adapted to O-H-LSD analysis for use in high-volume drug-testing laboratories. In addition, the possibility of significantly increasing the LSD detection time window by targeting this major LSD metabolite for analysis may influence other drug-free workplace programs to test for LSD.