Automated Extraction of Lysergic Acid Diethylamide (LSD) and N-demethyl-LSD from Blood, Serum, Plasma, and Urine Samples Using the Zymark RapidTrace™ with LC/MS/MS Confirmation
Journal of Forensic Sciences – May 01, 1998
Source: OpenAlex
Summary
A groundbreaking forensic procedure achieved over 95% recovery of lysergic acid diethylamide (LSD) from blood, serum, plasma, and urine samples. Utilizing the Zymark RapidTrace™ for automated solid-phase extractions, it confirmed LSD with a limit of quantitation at 0.05 ng/mL and a detection limit for both LSD and its metabolite, N-demethyl-LSD, at 0.025 ng/mL. The method demonstrated high precision, with a relative standard deviation of just 2.2% for within-run tests at 1.0 ng/mL, ensuring reliable results in analytical chemistry applications.
Abstract
Abstract A forensic procedure for the quantitative confirmation of lysergic acid diethylamide (LSD) and the qualitative confirmation of its metabolite, N-demethyl-LSD, in blood, serum, plasma, and urine samples is presented. The Zymark RapidTrace™ was used to perform fully automated solid-phase extractions of all specimen types. After extract evaporation, confirmations were performed using liquid chromatography (LC) followed by positive electrospray ionization (ESI+) mass spectrometry/mass spectrometry (MS/MS) without derivatization. Quantitation of LSD was accomplished using LSD-d3 as an internal standard. The limit of quantitation (LOQ) for LSD was 0.05 ng/mL. The limit of detection (LOD) for both LSD and N-demethyl-LSD was 0.025 ng/mL. The recovery of LSD was greater than 95% at levels of 0.1 ng/mL and 2.0 ng/mL. For LSD at 1.0 ng/mL, the within-run and between-run (different day) relative standard deviation (RSD) was 2.2% and 4.4%, respectively.