The Determination of Lysergide (LSD) in Urine by High-Performance Liquid Chromatography-Isotope Dilution Mass Spectrometry (IDMS)
Journal of Forensic Sciences – March 01, 1999
Source: OpenAlex
Summary
A groundbreaking method for confirming lysergic acid diethylamide (LSD) in urine has achieved a detection limit of 0.5 ng/mL, with potential improvement to 0.1 ng/mL. Utilizing isotope dilution mass spectrometry (IDMS) and a deuterated LSD analog as an internal standard, this approach enhances accuracy over traditional methods. The study validated the method's linearity up to 10 ng/mL and demonstrated its precision, marking a significant advancement in forensic drug analysis using gas chromatography–mass spectrometry and selected ion monitoring techniques.
Abstract
Abstract The use of isotope dilution mass spectrometry (IDMS) has been investigated for the forensic confirmation of lysergic acid diethylamide (LSD) in urine by LC-MS. The advantages of using a deuterated analog of LSD as an internal standard over methysergide are discussed. This study includes a comparison of the electrospray mass spectra of LSD, LSD-d3 and methysergide, and discusses the choice of suitable ions for use in selected ion monitoring (SIM) mode. An IDMS method is presented for the LC-MS confirmation of LSD in urine, with a limit of quantification (LOQ) of 0.5 ng/mL, reflecting the forensic requirement at this laboratory. Under some circumstances the LOQ can be improved to 0.1 ng/mL. This method is linear in the range tested (up to 10 ng/mL LSD in urine) and has been validated in terms of accuracy and precision.