Determination of lysergic acid diethylamide (LSD) in mouse blood by capillary electrophoresis/ fluorescence spectroscopy with sweeping techniques in micellar electrokinetic chromatography
Electrophoresis – March 01, 2003
Source: OpenAlex
Summary
Lysergic acid diethylamide (LSD) was successfully detected in mouse blood using advanced capillary electrophoresis and fluorescence spectroscopy techniques. After administering 0.1 mg of LSD to a 20 g mouse, concentrations reached 120 ng/mL at 20 minutes and 30 ng/mL at 60 minutes post-ingestion. This innovative approach utilized sodium dodecyl sulfate as a surfactant and employed methods like sweeping micellar electrokinetic chromatography to enhance on-line concentration and separation, demonstrating significant potential for applications in analytical chemistry and microfluidic technologies.
Abstract
Abstract The separation and on‐line concentration of lysergic acid diethylamide (LSD) in mouse blood was achieved by means of capillary electrophoresis/fluorescence spectroscopy using sodium dodecyl sulfate (SDS) as the surfactant. Techniques involving on‐line sample concentration, including sweeping micellar electrokinetic chromatography (sweeping‐MEKC) and cation‐selective exhaustive injection‐sweep‐micellar electrokinetic chromatography (CSEI‐sweep‐MEKC) were applied; the optimum on‐line concentration and separation conditions were determined. In the analysis of an actual sample, LSD was found in a blood sample from a test mouse (0.1 mg LSD fed to a 20 g mouse; ∼1/10 to the value of LD 50 ). As a result, 120 and 30 ng/mL of LSD was detected at 20 and 60 min, respectively, after ingestion of the doses.