Determination of Lysergic Acid Diethylamide (LSD) in Urine by Instrumental High-Performance Thin-Layer Chromatography
Journal of Analytical Toxicology – September 01, 1990
Source: OpenAlex
Summary
A groundbreaking high-performance thin-layer chromatographic (HPTLC) technique can detect lysergic acid diethylamide (LSD) in urine at levels below 1 microgram per liter. Utilizing a single-step alkaline extraction, this method offers a reliable alternative to traditional radioimmunoassay (RIA) procedures. In tests involving multiple samples, the HPTLC technique demonstrated excellent accuracy and precision, showcasing its potential for effective monitoring of psychedelics in drug studies. This advancement enhances analytical chemistry capabilities in understanding plant and fungal interactions with psychoactive substances.
Abstract
An instrumental high-performance thin-layer chromatographic (HPTLC) technique for the determination of lysergic acid diethylamide (LSD) in urine was developed. Before chromatographic separation, a single-step extraction with alkaline wash is performed. The procedure can detect less than 1 microgram LSD/L urine. Results of HPTLC determination are compared with those from a radioimmunoassay (RIA) procedure.