Determination of LSD in Urine With High-Performance Liquid Chromatography–Mass Spectrometry
Therapeutic Drug Monitoring – August 01, 2001
Source: OpenAlex
Summary
A groundbreaking method for detecting lysergic acid diethylamide (LSD) in urine achieves an impressive detection limit of 0.02 ng/mL. Utilizing high-performance liquid chromatography and mass spectrometry, this approach incorporates sample purification via organic solvent extraction. The calibration curve shows linearity from 0.05 to 10 ng/mL. Notably, within-day and between-day variations are minimal at 3.5% and 4.0%, respectively, while the extraction recovery rate stands at 91%. This method enhances analytical chemistry capabilities for LSD detection in routine testing.
Abstract
A rapid, specific, and sensitive high-performance liquid chromatography/mass spectrometry method has been developed for routine determination of lysergic acid diethylamide (LSD) in urine. It includes sample purification by extraction into an organic solvent and back-extraction to an acetate buffer, reversed-phase high-performance liquid chromatography, and detection with a single quadrupole mass spectrometer equipped with an atmospheric pressure ionization electrospray interface. Trideuterated LSD was used as internal standard. The limit of detection was 0.02 ng/mL and the calibration curve was linear from 0.05 to 10 ng/mL. Within-and between-day coefficients of variation were 3.5% and 4.0% respectively and extraction recovery was 91%.