Evaluation of the Cytotoxicity of Ayahuasca Beverages
Molecules – November 28, 2020
Source: OpenAlex
Summary
Ayahuasca, a traditional hallucinogenic beverage, has shown significant cytotoxic effects on dopaminergic cells. In a study involving N27 immortalized dopaminergic neurons, exposure to individual compounds and five different ayahuasca tea preparations led to a notable decrease in cell viability and protein levels. Specifically, both the alkaloids from Peganum harmala and the mixtures derived from traditional recipes demonstrated harmful effects. This groundbreaking analysis highlights the need to understand the biochemical impacts of ayahuasca and its components on neurological health.
Abstract
Ayahuasca is a beverage consumed at shamanic ceremonies and currently has gained popularity on recreational scenarios. It contains beta-carboline alkaloids and N,N-dimethyltryptamine, which possesses hallucinogenic effects. Only a few studies have elicited the psychoactive effects and the dose of such compounds on neurological dopaminergic cells or animals. In this work, we aimed to study the cytotoxic effects of these compounds present in ayahuasca beverages and on five different teas (Banisteriopsis caapi, Psychotria viridis, Peganum harmala, Mimosa tenuiflora and Dc Ab (commercial name)) preparations on dopaminergic immortalized cell lines. Moreover, a characterization of the derivative alkaloids was also performed. All the extracts were characterized by chromatographic systems and the effect of those compounds in cell viability and total protein levels were analyzed in N27 dopaminergic neurons cell line. This is the first article where cytotoxicity of ayahuasca tea is studied on neurological dopaminergic cells. Overall, results showed that both cell viability and protein contents decreased when cells were exposed to the individual compounds, as well as to the teas and to the two mixtures based on the traditional ayahuasca beverages.