Investigating the ability of the microbial model Cunninghamella elegans for the metabolism of synthetic tryptamines
Drug Testing and Analysis – November 21, 2018
Source: OpenAlex
Summary
Cunninghamella elegans effectively transforms tryptamines, with 63% of known phase I metabolites detected in its metabolism. This study examined four tryptamines, including the well-known N,N-Dimethyltryptamine (DMT), over 72 hours. Utilizing advanced liquid chromatography–mass spectrometry, researchers identified key metabolic pathways such as hydroxylation and demethylation. Notably, C. elegans produced unique metabolites not previously documented. These findings highlight the fungus's potential as a valuable model for studying the biochemistry of both natural and synthetic tryptamines, crucial for understanding their influence on brain disorders and behavior.
Abstract
Abstract Tryptamines can occur naturally in plants, mushrooms, microbes, and amphibians. Synthetic tryptamines are sold as new psychoactive substances (NPS) because of their hallucinogenic effects. When it comes to NPS, metabolism studies are of crucial importance, due to the lack of pharmacological and toxicological data. Different approaches can be taken to study in vitro and in vivo metabolism of xenobiotica. The zygomycete fungus Cunninghamella elegans ( C. elegans ) can be used as a microbial model for the study of drug metabolism. The current study investigated the biotransformation of four naturally occurring and synthetic tryptamines [ N,N‐ Dimethyltryptamine (DMT), 4‐hydroxy‐ N ‐methyl‐ N ‐ethyltryptamine (4‐HO‐MET), N,N ‐di allyl‐5‐methoxy tryptamine (5‐MeO‐DALT) and 5‐methoxy‐ N ‐methyl‐ N ‐isoporpoyltryptamine (5‐MeO‐MiPT)] in C. elegans after incubation for 72 hours. Metabolites were identified using liquid chromatography–high resolution–tandem mass spectrometry (LC–HR–MS/MS) with a quadrupole time‐of‐flight (QqTOF) instrument. Results were compared to already published data on these substances. C. elegans was capable of producing all major biotransformation steps: hydroxylation, N ‐oxide formation, carboxylation, deamination, and demethylation. On average 63% of phase I metabolites found in the literature could also be detected in C. elegans . Additionally, metabolites specific for C. elegans were identified. Therefore, C. elegans is a suitable complementary model to other in vitro or in vivo methods to study the metabolism of naturally occurring or synthetic tryptamines.