Use of Coconut Charcoal and Menthone-Thiosemicarbazone Polymer as Solid Phase Materials for the Determination of N,N-Dimethyltryptamine, Harmine, Harmaline, Harmalol, and Tetrahydroharmine in Ayahuasca Beverage by Liquid Chromatography-Tandem Mass Spectrometry
Journal of the Brazilian Chemical Society – January 01, 2018
Source: OpenAlex
Summary
A novel extraction method effectively identified key alkaloids in ayahuasca, including harmaline and harmine, using ultra-performance liquid chromatography-tandem mass spectrometry. Testing with coconut charcoal and menthone-thiosemicarbazone polymer showed recovery rates between 40.6% and 116.2%. Detection limits ranged from 0.003 to 0.016 μg mL -1, while quantification limits were between 0.009 and 0.043 μg mL -1. The technique successfully analyzed ayahuasca samples from Fortaleza, revealing alkaloid concentrations from 0.3 to 19.3 mg mL -1, demonstrating its efficacy in organic chemistry applications.
Abstract
An extraction method based on solid-phase extraction was developed to determine the major alkaloid components, N,N-dimethyltryptamine, harmine, harmaline, harmalol, and tetrahydroharmine, in ayahuasca using ultra-performance liquid chromatography-tandem mass spectrometry.Coconut charcoal and menthone-thiosemicarbazone polymer were tested as new adsorbent materials.Experiments carried out at levels of 1, 5, 10, 50, 100, and 250 μg mL -1 resulted in recoveries in the ranges 45.3-115.7%for menthone-thiosemicarbazone and 40.6-116.2%for coconut charcoal.The detection and quantification limits ranged from 0.003 to 0.016 μg mL -1 and from 0.009 to 0.043 μg mL -1 , respectively.The method developed was linear over the tested range (0.05-1.0 μg mL -1 ), with correlation coefficients ranging from 0.9969 to 0.9998.The technique was applied for analysis of the compounds in an ayahuasca sample collected from a religious group located in the city of Fortaleza, obtaining concentrations in the range from 0.3 to 19.3 mg mL -1 .