In vivometabolism ofα-methyltryptamine in rats: Identification of urinary metabolites
Xenobiotica – November 03, 2008
Source: OpenAlex
Summary
The metabolism of alpha-methyltryptamine (AMT) reveals significant insights into psychoactive compounds. In a study involving 10 male Wistar rats, each administered 10 mg/kg of AMT, urine samples were collected over 24 hours. Following enzymatic hydrolysis and liquid-liquid extraction, gas chromatography–mass spectrometry identified four key metabolites: 2-oxo-AMT, 6-hydroxy-AMT, 7-hydroxy-AMT, and 1'-hydroxy-AMT. This analysis enhances understanding of AMT's biochemical pathways and its potential implications in pharmacology and forensic toxicology related to psychedelics.
Abstract
1. The in vivo metabolism of alpha-methyltryptamine (AMT), a psychoactive tryptamine analogue, was studied in rats. 2. Male Wistar rats were administered 10 mg kg(-1) AMT orally and 24-h urine fractions were collected. After enzymatic hydrolysis of the urine sample, the metabolites were extracted by liquid-liquid extraction and analysed by gas chromatography/mass spectrometry (GC/MS). 3. 2-Oxo-AMT, 6-hydroxy-AMT, 7-hydroxy-AMT and 1'-hydroxy-AMT were detected as metabolites of AMT.