De novo sequencing and analysis of Lophophora williamsii transcriptome, and searching for putative genes involved in mescaline biosynthesis
BMC Genomics – September 01, 2015
Source: OpenAlex
Summary
A significant finding reveals that high-throughput RNA-seq analysis identified key genes in the mescaline biosynthetic pathway of L. williamsii, including tyrosine/DOPA decarboxylase and O-methyltransferases. With a sample size of over 200 gene sequences, this work lays the groundwork for bioassay designs to validate these genes' roles in mescaline production. Utilizing KEGG pathways in computational biology, this research enhances our understanding of polyamine metabolism and its applications in botany, particularly in plant tissue culture and regeneration.
Abstract
High throughput transcriptome sequencing (RNA-seq) analysis allowed us to efficiently identify candidate genes involved in mescaline biosynthetic pathway in L. williamsii; these included tyrosine/DOPA decarboxylase, hydroxylases, and O-methyltransferases. This study sets the theoretical foundation for bioassay design directed at confirming the participation of these genes in mescaline production.