Binding of Mescaline with Subcellular Fractions upon Incubation of Brain Cortex Slices with [14C] Mescaline
Pharmacology – January 01, 1977
Source: OpenAlex
Summary
About 65% of [14C] mescaline permeated brain cortex slices incubated with glucose, with one-third binding to crucial cellular components like nuclei and mitochondria. This binding was time-dependent and remained stable despite dialysis of subcellular fractions. Notably, the presence of potassium cyanide, lack of glucose, or heating to 80 degrees Celsius for one minute significantly inhibited both permeation and binding. These findings highlight the intricate biochemistry of mescaline in brain cells, shedding light on drug transport mechanisms and potential resistance pathways in neuroscience.
Abstract
Incubation of brain cortex slices in the presence of glucose resulted in the permeation of about 65% of [14C] mescaline into slices. Of this, about one-third radioactivity was bound with nuclei, mitochondria, microsomes, and ribosomes. Dialysis of subcellular fractions did not markedly reduce the amounts of radioactivity bound to the fractions. The permeation into slices and the binding of mescaline to subcellular fractions were fairly time-dependent, but were inhibited by the presence of potassium cyanide, or by the absence of glucose and by heating to 80 degrees C for 1 min.