Effects of ibogaine per os application on redox homeostasis in rat liver and erythrocytes
Teodora Vidonja Uzelac, Nikola Tatalović, Milica Mijović, Gordana Koželj, Aleksandra Nikolić‐kokić, Zorana Oreščanin-dušić, Mara Bresjanac, Duško Blagojević
Archives of Biological Sciences December 5, 2018 Peer reviewed DOI: 10.2298/abs180918055v via OpenAlex
Summary
Ibogaine, given as a single oral dose of 1 or 20 mg/kg, affects oxidative stress in rats but does not change the activity of key antioxidant enzymes in the liver and erythrocytes after 6 and 24 hours. While hepatic xanthine oxidase activity increased with the higher dose, indicating faster adenosine turnover, TBARS levels showed mild oxidative stress in the lower dose group. The highest glycogenolytic activity was observed in hepatocytes at 24 hours post-administration of 20 mg/kg ibogaine.
Study at a glance
| Population | rats |
|---|---|
| Key finding | Ibogaine treatment influenced hepatic redox homeostasis but did not significantly alter antioxidant enzyme activities after 6 and 24 hours. |
Abstract
Ibogaine, administered as a single oral dose (1-25 mg/kg body weight), has been used as an addiction-interrupting agent. Its effects persist for up to 72 h. Ex vivo results showed that ibogaine induced cellular energy consumption and restitution, followed by increased reactive oxygen species production and antioxidant activity. Therefore, the aim of this work was to explore the effect of a single oral dose of ibogaine (1 or 20 mg/kg body weight) on antioxidative defenses in rat liver and erythrocytes. Six and 24 h after ibogaine administration, histological examination showed glycogenolytic activity in hepatocytes, which was highest after 24 h in animals that received 20 mg/kg ibogaine. There were no changes in the activities of superoxide dismutases, catalase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase in the liver and erythrocytes after ibogaine treatment, regardless of the dose. Hepatic xanthine oxidase activity was elevated in rats that received 20 mg/kg compared to the controls (p<0.01), suggesting faster adenosine turnover. TBARS concentration was elevated in the group treated with 1 mg/kg after 24 h compared to the controls (p<0.01), suggesting mild oxidative stress. Our results show that ibogaine treatment influenced hepatic redox homeostasis, but not sufficiently to remodel antioxidant enzyme activities at 6 and 24 h post-ibogaine application.