Identification of 2C-B in Hair by UHPLC-HRMS/MS. A Real Forensic Case.
Toxics – July 15, 2021
Source: PubMed
Summary
Identifying emerging hallucinogens like 2C-B, often called "pink cocaine," in forensic samples has been a challenge. A new UHPLC-HRMS/MS method, utilizing an Orbitrap system, successfully detected this NPS (new psychoactive substance), also known as nexus, in hair from a real case. This advanced technique establishes a powerful nexus for forensic analysis.
Abstract
The analysis of drugs of abuse in hair and other biological matrices of forensic interest requires great selectivity and sensitivity. This has been traditionally achieved through target analysis, using one or more analytical methods that include different preanalytical stages, and more complex procedures followed by toxicological laboratories. There is no exception with 2C-series drugs, such as 2C-B, a new psychoactive substance (NPS), which use has emerged and significantly increased, year by year, in the last decades. Continuously new analytical methods are required to selectively detect and identify these new marketed substances at very low concentrations. In this case report, one former case of a polydrug consumer (charged of a crime against public health in Spain) was reanalyzed in hair matrix. In this reanalysis, 2C-B has been positively detected and identified using liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS/MS). The most selective analytical UHPLC-HRMS/MS method alongside a universal and simpler pretreatment methodology has opened up more possibilities for the detection of substances of different chemical structure and optimization of different HRMS/MS detection approaches allowing the identification of 2-CB in the hair of a real forensic case.