Quantitation of psilocin in human plasma by high-performance liquid chromatography and electrochemical detection: comparison of liquid–liquid extraction with automated on-line solid-phase extraction
Journal of Chromatography B Biomedical Sciences and Applications May 1, 1998 Hiltrud Lindenblatt, Edda Krämer, Petra Holzmann-Erens et al. 92 citations
Two modifications of an HPLC-ED method for measuring psilocin, the active metabolite of psilocybin, in human plasma were developed and compared. One used liquid-liquid extraction (LLE) and the other automated on-line solid-phase extraction (on-line SPE). Both methods had a limit of quantitation of 10 ng/ml psilocin and showed no significant difference in standard deviation (LLE 1.82%, on-line SPE 1.13%) or analytical results. On-line SPE required less manual effort, smaller plasma volumes (400 microl versus 2 ml), and achieved nearly 100% recovery of psilocin (LLE 88%). Both methods were rapid, simple, and reliable, and were successfully used to quantify psilocin in plasma samples from healthy volunteers after oral administration of 0.2 mg psilocybin per kg body mass.