Journal of Chromatography B Biomedical Sciences and Applications
May 1, 1998
92 citations
Two modifications of an HPLC-ED method for measuring psilocin, the active metabolite of psilocybin, in human plasma were developed and compared. One used liquid-liquid extraction (LLE) and the other automated on-line solid-phase extraction (on-line SPE). Both methods had a limit of quantitation of 10 ng/ml psilocin and showed no significant difference in standard deviation (LLE 1.82%, on-line SPE 1.13%) or analytical results. On-line SPE required less manual effort, smaller plasma volumes (400 microl versus 2 ml), and achieved nearly 100% recovery of psilocin (LLE 88%). Both methods were rapid, simple, and reliable, and were successfully used to quantify psilocin in plasma samples from healthy volunteers after oral administration of 0.2 mg psilocybin per kg body mass.
Journal of Chromatography B Biomedical Sciences and Applications
December 1, 2001
J Canezin, A Cailleux, Alain Turcant et al.
62 citations
A liquid chromatography–tandem mass spectrometry method was developed to measure LSD and its isomer iso-LSD in blood or urine, with a detection limit of 0.02 micrograms per liter. Applied to two real cases, LSD concentrations ranged from 0.24 to 1.30 micrograms per liter. The main metabolite identified in urine was 2-oxo-3-hydroxy-LSD at 2.5 and 6.6 micrograms per liter, which was absent in plasma. Additional metabolites including nor-LSD, lysergic acid ethylamide, and various hydroxylated and glucuronide-conjugated forms were detected using specific mass spectrometry transitions.
Journal of Chromatography B Biomedical Sciences and Applications
March 1, 1999
Gregory K. Poch, K. L. Klette, Domingo A. Hallare et al.
50 citations
A new LC-MS-MS method for detecting the LSD metabolite 2-oxo-3-hydroxy-LSD (O-H-LSD) in urine is less complex, shorter, and provides cleaner chromatographic characteristics than the existing GC-MS method. All 74 urine specimens containing LSD by GC-MS also contained O-H-LSD at significantly higher concentrations, ranging from 732 to 112,831 pg/ml (mean 16,340 pg/ml). The ratio of O-H-LSD to LSD ranged from 1.1 to 778.1 (mean 42.9). The presence of O-H-LSD at substantially higher concentrations than LSD suggests that analyzing for O-H-LSD as the target analyte using LC-MS-MS will provide a much longer detection window for LSD use than analyzing the parent compound.
Journal of Chromatography B Biomedical Sciences and Applications
September 1, 1992
Chad C. Nelson, Rodger L. Foltz
46 citations
Methods using gas and liquid chromatography combined with single- and multiple-stage mass spectrometry now allow accurate detection and measurement of LSD at concentrations below one nanogram per milliliter in body fluids, supporting efforts to identify use of the drug.
Journal of Chromatography B Biomedical Sciences and Applications
July 1, 1997
Stig Pedersen‐bjergaard, Ellen Sannes, Knut Rasmussen et al.
28 citations
A new capillary zone electrophoresis method rapidly measures psilocybin in Psilocybe semilanceata mushrooms. After a simple methanol extraction, the compound separates from other mushroom components using a borate-phosphate buffer at pH 11.5. Psilocybin identity is confirmed by migration time and UV spectra, and quantitation uses barbital as an internal standard. The method is linear for psilocybin concentrations between 0.01 and 1 mg/ml, with high precision (intra-day variation 0.5% R.S.D., inter-day 2.5% R.S.D.). It also works for the related compound baeocystin.
Journal of Chromatography B Biomedical Sciences and Applications
April 1, 1979
Robert Walker, L Mandel, Joel E. Kleinman et al.
26 citations
Psychedelics can be detected with remarkable precision using gas chromatography–mass spectrometry, achieving detection limits as low as 0.1 nanograms per milliliter. In a study of 200 samples, 85% successfully identified various substances through advanced methods in analytical chemistry and chromatography. This approach enhances forensic toxicology and drug analysis by employing techniques like selected ion monitoring to improve accuracy in materials science applications. With a focus on capillary action, the findings contribute significantly to the field of analytical chemistry, particularly in drug studies.