Determination of LSD and its metabolites in human biological fluids by high-performance liquid chromatography with electrospray tandem mass spectrometry
Journal of Chromatography B Biomedical Sciences and Applications December 1, 2001 J Canezin, A Cailleux, Alain Turcant et al. 62 citations
A liquid chromatography–tandem mass spectrometry method was developed to measure LSD and its isomer iso-LSD in blood or urine, with a detection limit of 0.02 micrograms per liter. Applied to two real cases, LSD concentrations ranged from 0.24 to 1.30 micrograms per liter. The main metabolite identified in urine was 2-oxo-3-hydroxy-LSD at 2.5 and 6.6 micrograms per liter, which was absent in plasma. Additional metabolites including nor-LSD, lysergic acid ethylamide, and various hydroxylated and glucuronide-conjugated forms were detected using specific mass spectrometry transitions.