Determination of psilocybin in Psilocybe semilanceata by capillary zone electrophoresis
Journal of Chromatography B Biomedical Sciences and Applications July 1, 1997 Stig Pedersen‐bjergaard, Ellen Sannes, Knut Rasmussen et al. 28 citations
A new capillary zone electrophoresis method rapidly measures psilocybin in Psilocybe semilanceata mushrooms. After a simple methanol extraction, the compound separates from other mushroom components using a borate-phosphate buffer at pH 11.5. Psilocybin identity is confirmed by migration time and UV spectra, and quantitation uses barbital as an internal standard. The method is linear for psilocybin concentrations between 0.01 and 1 mg/ml, with high precision (intra-day variation 0.5% R.S.D., inter-day 2.5% R.S.D.). It also works for the related compound baeocystin.