Optimization and immunological characterization of a photochemically coupled lysergic acid diethylamide (LSD) immunogen
Bioconjugate Chemistry January 1, 1998 Sarah Kerrigan, Donald. E. Brooks 3 citations
A photoreactive linker was used to attach lysergic acid diethylamide (LSD) to a carrier protein, keyhole limpet hemocyanin, at multiple sites on the drug. About 35 drug molecules were attached per protein when equal amounts of both were used. Reaction efficiency was severely reduced by buffer components or water. Excess linker, pH, irradiation of the dry matrix without buffer, and the drug-to-protein ratio during photolysis strongly affected efficiency. Antibody specificity data suggested that the drug attached to the protein at positions N1 and N6 of LSD, consistent with the reactivity of the photogenerated aryl nitrene.